Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific CTL and Tfh responses to ZVL in the VZV-specific flow cytometry subset of the primary cohort ( n = 95) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques: Flow Cytometry

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline and peak immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Expression of FOXP3 and inhibitory ligands on CD4+ CD25+ CD127− Treg. Data were derived from 6 adults of all ages. The parent population is indicated on the abscissa. Horizontal bars indicate paired comparisons and P values.

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques: Expressing, Derivative Assay

Journal: iScience

Article Title: Defects in NK cell immunity of pediatric cancer patients revealed by deep immune profiling

doi: 10.1016/j.isci.2024.110837

Figure Lengend Snippet:

Article Snippet: Anti-CD25 (clone 2A3, conjugated to 149Sm) , Standard BioTools , Cat#3149010B.

Techniques: Purification, Clinical Proteomics, Recombinant, Blocking Assay, Staining, Saline, Mass Cytometry, Software, Cytometry

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapy

doi: 10.1007/s00262-025-04290-y

Figure Lengend Snippet: DCN increases the efficacy of anti-PD1 treatment. A - C C57BL/6 mice were implanted with DCN-overexpressed or control KP141 tumor cells with treatment of IgG isotype control or anti-PD1 monoclonal antibody ( n = 8 from 4 mice per group). The experimental schematic diagram ( A ), tumor volume ( B ), and tumor weight ( C ). D Immunofluorescent staining of CD8 and DAPI in subcutaneous KP141 tumors with or without DCN overexpression. Quantification of CD8 + cells per field is shown on the right ( n = 10). E The relative expression levels of Dcn in responders and non-responders to ICB therapy were analyzed across multiple mouse tumor models using the analytical platform at tismo.cistrome.org. F Kaplan–Meier survival analysis of OS in patients with ICB therapy. Two-sided log-rank test. G Kaplan–Meier survival curves for OS in patients with anti-PD1 therapy. Data from kmplot.com. Two-sided log-rank test

Article Snippet: In vivo anti-PD1 treatment: anti-mouse PD1 (BE0273, BioXcell) and anti-rat IgG2a isotype control (BE0089, BioXcell).

Techniques: Control, Staining, Over Expression, Expressing

Journal: Frontiers in Immunology

Article Title: Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells

doi: 10.3389/fimmu.2018.00922

Figure Lengend Snippet: Irradiation stimulates immune activation in Jurkat cells and peripheral blood lymphocytes (PBL). FACS analysis of CD25 surface expression on Jurkat cells and CD3-positive PBL (A) following irradiation with a dose of 1.25, 2, and 5 Gy. Stimulation with 25 µl/ml CD3/CD28/CD2 T-cell activator (Act.) in Jurkat cells or mock-irradiated cells served as controls ( N = 3). In PBLs, an activator could not be applied due to inference with the CD3 stimulus. Quantification of interleukin (IL)-2 (B) and interferon-γ (IFNγ) (C) mRNA expression by quantitative real-time PCR in Jurkat cells and PBL at 24 h after irradiation with a dose of 1.25, 2, or 5 Gy. Stimulation with 25 µl/ml CD3/CD28/CD2 T-cell activator (Act.) or mock-irradiated cells served as controls ( N = 2). Data are represented as mean + SD. Student’s t -test compared activator-treated or irradiated cells with non-irradiated controls; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Next, cells were stained with fluorochrome-conjugated mAb targeting CD3 (CD3-PerCP-Cy5.5 clone SK7; Becton Dickinson, Heidelberg, Germany) and CD25 (BV510 Mouse anti human CD25 clone 2A3, Becton Dickinson) and subjected to multicolor flow cytometry using a CytoFlexS cytometer (Beckman Coulter, Krefeld, Germany).

Techniques: Irradiation, Activation Assay, Expressing, Real-time Polymerase Chain Reaction

Journal: eLife

Article Title: A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection

doi: 10.7554/elife.65762

Figure Lengend Snippet: Figure 1. Neutrophil depletion reduces disease severity after HSV-2 vaginal infection. Female C57BL/6J mice were treated with depot medroxyprogestrone (DMPA) and inoculated intravaginally (ivag) with 5000 plaque forming units (PFU) of herpes simplex virus-2 (HSV-2). One day prior to HSV-2 inoculation, mice were injected intraperitoneally (i.p.) with 500 mg of rat IgG2a isotype control or anti-Ly6G monoclonal antibody (mAb). (A) Plots show gating strategy to identify neutrophils in the vagina. Numbers in plots refer to percent of parent population for gated cells (d0, d2: n = 6, d2: n = 9, d6 isotype: n = 8, d6 anti-Ly6G: n = 10). Depletion was confirmed by flow cytometry in the vagina on the indicated days. (B) Inflammation scores over the first 7 d.p.i. of mice treated with anti-Ly6G antibody (n = 25) or isotype control (n = 23). Mice showed no signs of disease prior to 4 d.p. i. (C) Histology of the vagina (top) or genital skin (bottom) at 6 d.p.i. from isotype control (left) or anti-Ly6G antibody-treated mice (right). Red arrows point to areas of epithelial denuding or damage, black arrows denote the basement membrane. (D) Infectious virus as measured by plaque assay in vaginal washes collected daily (both groups d1: n = 22, d2: n = 28, d3: n = 15, d4: n = 16, d5: n = 19, d6: n = 6, d7: n = 8). (E) Viral load was measured in homogenized vaginal tissue collected at 7 d.p.i. from the indicated groups (n = 8). Data in A and E are pooled from two independent experiments, and data in B and D are pooled from two to four independent experiments. Data in C is representative of two independent experiments. Bars in B show median with interquartile range. Horizontal bars in A, D, and E show mean. Scale bars show 50 mm. Statistical analysis was performed by two-way ANOVA on log-transformed data with Bonferroni’s multiple comparisons test (A), repeated measures two-way ANOVA with Geisser-Greenhouse correction and Bonferroni’s multiple comparisons test (B) repeated measures two-way ANOVA with Bonferroni’s multiple comparison’s test (D) or Mann-Whitney test (E). *p<0.05, **p<0.01, ***p<0.005, ****p<0.001. Raw values for each biological replicate, epsilon values, and specific p values are provided in Figure 1—source data 1. The online version of this article includes the following source data and figure supplement(s) for figure 1:

Article Snippet: For experiments in which IL-18 was neutralized, mice were treated intravaginally with 100 mg of anti-IL-18 antibody (clone YIGIF74-1G7) or rat IgG2a isotype control (clone 2A3) (BioXCell) on days 3–5 after infection.

Techniques: Infection, Virus, Injection, Control, Flow Cytometry, Membrane, Plaque Assay, Transformation Assay, MANN-WHITNEY